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Configuration Generator
Config Generator
Brown Lab Single Cell RNA Sequencing
Directory Info
Alignment Output Directory:
Path to parent dir of .h5 data
Merge Samples
Merge Samples:
Merge all samples without integration
Samples
Add Sample
Filter Options
Filter Out Mitochondrial:
Remove cells above cutoff percentage
Regress Mitochondrial Variation:
Regress out Mitochondrial Variation with SCTransform
Percent Mitochondrial:
Cutoff percentage of features that come from Mitochondrial RNA
nCount RNA Upper:
Upper UMI count limit
nCount RNA Lower:
Lower UMI count limit
nFeature RNA Upper:
Upper unique feature count limit
nFeature RNA Lower:
Lower unique feature count limit
Dimensional Reduction Settings
Run UMAP:
Run tSNE:
PCA Component Count:
Init Seurat Settings
Min Cells:
Min Features:
Featureplot Settings
Add Group
Generate JSON